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To date, this study presents the most extensive and wide diversity collection of mycoviruses infecting the necrotrophic fungus B. cinerea . Ana Ruiz-Padilla , Julio Rodríguez-Romero , Irene Gómez-Cid , Davide Pacifico , María A. Ayllón 11 May 2021 Research Article Dissecting Cellular Function and Distribution of β-Glucosidases in Trichoderma reesei Although β-glucosidases play an important role in fungal cellulase induction and production, our current understanding does not provide a global perspective on β-glucosidase function. This work comprehensively studies all the β-glucosidases regarding their effect on cellulase production and their cellular distribution and secretion. Ai-Ping Pang , Haiyan Wang , Yongsheng Luo , Zihuayuan Yang , Zhiyu Liu , Zhao Wang , Bingzhi Li , Song Yang , Zhihua Zhou , Xiaolin Lu , Fu-Gen Wu , Zuhong Lu , Fengming Lin 11 May 2021 Research Article Controls on Interspecies Electron Transport and Size Limitation of Anaerobically Methane-Oxidizing Microbial Consortia Anaerobic oxidation of methane is a globally important, microbially mediated process reducing the emission of methane, a potent greenhouse gas. Xiaojia He , Grayson L. Chadwick , Christopher P. Kempes , Victoria J. Orphan , Christof Meile 11 May 2021 Research Article The DnaK/DnaJ Chaperone System Enables RNA Polymerase-DksA Complex Formation in Salmonella Experiencing Oxidative Stress DksA was discovered 30 years ago in a screen for suppressors that reversed the thermosensitivity of Escherichia coli mutant strains deficient in DnaK/DnaJ, raising the possibility that this chaperone system may control DksA function. Since its serendipitous discovery, DksA has emerged as a key activator of the transcriptional program called the stringent response in... Ju-Sim Kim , Lin Liu , Andrés Vázquez-Torres 11 May 2021 Minireview Positioning the Model Bacterial Organelle, the Carboxysome Bacterial microcompartments (BMCs) confine a diverse array of metabolic reactions within a selectively permeable protein shell, allowing for specialized biochemistry that would be less efficient or altogether impossible without compartmentalization. BMCs play critical roles in carbon fixation, carbon source utilization, and pathogenesis. Joshua S. MacCready , Anthony G. Vecchiarelli 11 May 2021 Research Article Novel Role for ESCRT-III Component CHMP4C in the Integrity of the Endocytic Network Utilized for Herpes Simplex Virus Envelopment Cellular transport pathways play a fundamental role in secretion and membrane biogenesis. Enveloped viruses exploit these pathways to direct their membrane proteins to sites of envelopment and, as such, are powerful tools for unraveling subtle activities of trafficking factors, potentially pinpointing therapeutic targets. Tiffany Russell , Jerzy Samolej , Michael Hollinshead , Geoffrey L. Smith , Joanne Kite , Gillian Elliott 11 May 2021 Research Article The SARS-CoV-2 Transcriptome and the Dynamics of the S Gene Furin Cleavage Site in Primary Human Airway Epithelia Polarized human airway epithelia at an air-liquid interface (HAE-ALI) are an in vitro model that supports efficient infection of SARS-CoV-2. The spike (S) protein of SARS-CoV-2 contains a furin cleavage site (FCS) at the boundary of the S1 and S2 domains which distinguishes it from SARS-CoV. Wei Zou , Min Xiong , Siyuan Hao , Elizabeth Yan Zhang , Nathalie Baumlin , Michael D. Kim , Matthias Salathe , Ziying Yan , Jianming Qiu 11 May 2021 Editor’s Pick Research Article An Engineered Receptor-Binding Domain Improves the Immunogenicity of Multivalent SARS-CoV-2 Vaccines All available vaccines for coronavirus disease 2019 (COVID-19) express or deliver the full-length SARS-CoV-2 spike (S) protein. We show that this antigen is not optimal, consistent with observations that the vast majority of the neutralizing response to the virus is focused on the S-protein receptor-binding domain (RBD). Yan Guo , Wenhui He , Huihui Mou , Lizhou Zhang , Jing Chang , Shoujiao Peng , Amrita Ojha , Rubens Tavora , Mark S. Parcells , Guangxiang Luo , Wenhui Li , Guocai Zhong , Hyeryun Choe , Michael Farzan , Brian D. Quinlan 11 May 2021 Research Article Functional and Comparative Analysis of Centromeres Reveals Clade-Specific Genome Rearrangements in Candida auris and a Chromosome Number Change in Related Species Candida auris , the killer fungus, emerged as different geographical clades, exhibiting multidrug resistance and high karyotype plasticity. Chromosomal rearrangements are known to play key roles in the emergence of new species, virulence, and drug resistance in pathogenic fungi. Aswathy Narayanan , Rakesh Netha Vadnala , Promit Ganguly , Pavitra Selvakumar , Shivaprakash M. Rudramurthy , Rajendra Prasad , Arunaloke Chakrabarti , Rahul Siddharthan , Kaustuv Sanyal 11 May 2021 Research Article Manure Microbial Communities and Resistance Profiles Reconfigure after Transition to Manure Pits and Differ from Those in Fertilized Field Soil The addition of dairy cow manure—stored in manure pits—to field soil has the potential to introduce not only organic nutrients but also mammalian...